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The 425 dichroic mirror works at 45° incidence: visible light above 425 nm (mainly blue, green and red) is highly transmitted while light at or below 425 nm is highly reflected — a sharp spectral conversion at the 425 nm point. In fluorescence technology it efficiently separates excitation light from emission fluorescence, ensuring high-SNR detection; it is one of the core elements of fluorescence microscopes, flow cytometers and fluorescence spectrometers.









